基本情報/Basic Information

開講科目名 ／Course	分析技術法Ｃ／Analytical Methodology C
時間割コード ／Course Code	A242000005
ナンバリングコード ／Numbering Code
開講所属 ／Course Offered by	農学生命科学研究科／
曜日コマ ／Day, Period	他
開講区分 ／Semester offered	集中
単位数 ／Credits	1.0
学年 ／Year	1,2
主担当教員 ／Main Instructor	橋本 勝／HASHIMOTO MASARU
科目区分 ／Course Group	大学院（修士課程）
教室 ／Classroom
必修・選択 ／Required/Elective
授業形式 ／Class Format
メディア授業 ／Media lecture	－

担当教員情報/Instructor Information

教員名 ／Instructor	教員所属名 ／Faculty/Department
橋本 勝／HASHIMOTO MASARU	農学生命科学部／

難易度（レベル） ／Level	Level 5
対応するＤＰ ／DP	DP1
授業としての具体的到達目標 ／Concrete arrival target as the class	〇Understand the mechanism of mass spectrometry, and acquire the skills to apply this technology to structural and quantitative analyses. 〇Laern the skill of the structural analysis of compounds by performing actual measurements using a mass spectrometer.
授業の概要 ／Summary of the class	This course will teach you how to operate mass spectrometers and analyze the structures required as an engineer with a master's degree. Mass spectrometry is widely used for product quality control in the food and pharmaceutical industries and forensic and environmental investigations. This technology is essential in many fields.
授業の内容予定 ／Contents plan of the class	The detailed schedule will be announced. 1: What is the method of measuring mass? Principle of the mass spectrometer, characteristics of various ionization methods, high-resolution measurement, and isotope distribution analysis Part 2: Principles and features of GC-MS: Principle and features of LC-ESI-IT-TOF-MS, principle of CID fragmentation of peptides and its analysis, and deconvolution and protein mass analysis in LC-ESI-IT-TOF-MS High sensitivity measurement using nano LC-ESI-IT-TOF-MS Part 3: Principles and features of MALDI-TOF-MS Part 4: Overview of the instrument Part 5: Starting up the instrument and checking the program Part 6: Basic measurements Part 7: Applied measurement In lectures 4 to 7, students will select one of the following measurement methods and practice it. 1) Measurement using GC-MS (volatile molecules, fragrances) 2) Measurement using LC-ESI-IT-TOF-MS (secondary metabolites, peptides) (3) Measurement using MALDI-TOF-MS (protein) 8th analysis, discussion of analysis results
成績評価方法及び採点基準 ／A scholastic evaluation method and marking standard	Attitude, understanding of spectrum analysis (50%), and report (50%) will be evaluated.
予習及び復習等の内容 ／Contents such as preparations for lessons and the review	Please read about mass spectrometry in your organic chemistry textbook before the class.
教材・教科書 ／The teaching materials, textbook	志田保夫ら著「これならわかるマススペクトロメトリー」化学同人
参考文献 ／bibliography	Spectrometric Identification of Organic Compounds 8th Edition by Robert M. Silverstein, Francis X. Webster, David J. Kiemle, David L. Bryce
留意点・予備知識 ／Point to keep in mind, back ground	You will need to do some simple calculations. Please bring a calculator.
授業内容に関する質問・疑義等 ／Question, doubt about class contents	Monday 12:00-14:00 (Hashimoto). Tuesday 16:00-17:00 (Sakamoto). However, he is available as long as he is in his office.
Eメールアドレス・HPアドレス ／E-mail address, HP address	Hashimoto: e-mail: hmasaru[at]hirosaki-u.ac.jp home page URL: http://nature.cc.hirosaki-u.ac.jp/lab/2/biochem/yuki/ Sakamoto：sakamok[at]hirosaki-u.ac.jp
学問分野1（主学問分野） ／Discipline 1	G43:分子レベルから細胞レベルの生物学およびその関連分野
学問分野2（副学問分野） ／Discipline 2	E37:生体分子化学およびその関連分野
学問分野3（副学問分野） ／Discipline 3	H49:病理病態学、感染・免疫学およびその関連分野
地域志向科目 ／Local intention subject	なし
授業形態・授業方法 ／Class form, class method	The first half will be in lecture format and the second half will be in practice.
科目ナンバー ／The subject number
メディア授業による著作物利用の有無について ／Whether or not copyrighted works are used in media classes	無／Nothing
その他 ／Others	None in particular.

No.	回（日時） ／Time (date and time)	主題と位置付け（担当） ／Subjects and instructor's position	学習方法と内容 ／Methods and contents	備考 ／Notes
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